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1	ABS-386	Biology
Isolation and Identification of Potential Probiotics Bacteria from Intestinal of Anguilla bicolor Peristiwati*, Yayan Sanjaya **and Canthika Trinaya Department of Biology, Universitas Pendidikan Indonesia, Bandung, Indonesia Abstract Sidat is high selling price fish in international market. This is can causes sidat fish to be categorized as a source earners for the country. Probiotics are live bacteria promoted healthy in fish. Probiotics can also produce bacteriocins is usefull against several strains of pathogenic bacteria. This research aimed to isolated of probiotic bacteria in Anguila bicolor intestine and determine the characteristics of potential probiotics bacteria. The steps was taking sample of small intestine by dissect the stomach of Anguilla bicolor and taking out the digesta of small intestine and then dilution techniques were performed with pour plate and streak plate methods, Gram staining, Biochemical Tests and making Bacteria Growth Curve. To get the ability as probiotic bacteria by using a few test such as the ability in low pH and bile salt. The test of antagonistic ability to pathogen bacteria was used Staphylococcus aureus and Pseudomonas aeruginosa. The result shows that three isolates of Gram positive and have a rod shape. The three isolates characterized by biochemical tests belong to the genus Lactobacillus. They were able to grow and survive on a medium which has a pH between 3-6.5 and a medium of 0.05% and 0,1% synthetic bile salt. From the result of the antagonistic ability test show that all isolates have the ability to produce antimicrobial that are bacteriosida against bacterial growth of Staphylococcus aureus and Pseudomonas aeruginosa Keywords: Anguilla bicolor, Probiotics, Lactobacillus. bacteriosida PermaLink | Plain Format | Corresponding Author (Peristiwati Peristiwati)

2	ABS-394	Biology
THE EFFECT OF TURMERIC (Curcuma Xanthorrhiza) ON REPRODUCTIVE ASPECT OF MALE MICE (Mus musculus) SWISS WEBSTER Didik Priyandoko1, Hernawati1, Fitria Rachmayati1 Study Program of Biology, Departemen Pendidikan Biologi, Faculty of mathematics and science education,, Universitas Pendidikan Indonesia Abstract Turmeric rhizome (Curcuma xanthorrhiza) containing various chemical compounds including curcumin, protein, starch and essential oils are known to degrade cell proliferation. The aim of the study aims was to determine the effect of turmeric extract on reproductive aspects of mice (Mus musculus) male. The subjects were 24 male mice, and divided into 4 groups (control, 140 mg / kg, 280 mg / kg and 700 mg / kg). Mice were treated by gavage for 30 days after the previous acclimatization for 7 days. Samples taken from the epididymal sperm in mice and testing the number, motility and sperm abnormalities. While testicular taken to get the incision and measure the weight of the seminiferous tubules of the testes. The data obtained were analyzed with the Kruskal Wallis and Mann Whitney (p <0.05) with a 95% confidence level. The results showed that turmeric extract for 30 days significantly influence the decrease in testicular weight, but no significant effect on sperm count, motility, abnormalities and testicular histology incision. Keywords: Curcuma xanthorrhiza, Semen Morphology, Testicular Morphology, Mice PermaLink | Plain Format | Corresponding Author (Didik Priyandoko)

3	ABS-178	Biology
THE OPTIMIZATION OF HUMAN BLOOD AGAR (HBA) FOR STREPTOCOCCUS PNEUMONIAE GROWTH Anita Oktari, Noviana Vanawati, Ulva Indah Kurniawati Department of Medical Analyst, School of Analyst Bakti Asih, Bandung Indonesia Abstract Sheep Blood Agar (SBA) is a standard media for microbiology examination. The difficulty to get sheep blood in tropical and developing countries causes an obstacle in culturing Streptococcus pneumoniae. In other side, the usage of Human Blood Agar (HBA) were not recommended for culturing Streptococcus pneumoniae. The purpose of this experimental is to get the alternative media from HBA to improve the growth of Streptococcus pneumoniae. Methods experimental study with True experimental post test only design. Colony of Streptococcus pneumoniae that had been checked purity were planted on HBA media and SBA media. Observation was done in 24 hours and 48 hours, comprising of colony diameters, hemodygest diameters, and the characteristics colony. Results after 24 hours, the mean of Streptococcus pneumoniae colony diameters on SBA media were not significantly different (0,8 mm) with HBA media (0,6 mm). On the 48 hours observation, the mean of colony diameters on HBA media (1,5 mm) were narrower than those on SBA media (1,8 mm). After 24 hours, the mean of Streptococcus pneumoniae hemolysis diameters, on SBA media (1,2 mm) were significantly different with HBA media (0,6 mm). After the 48 hours observation, hemolysis diameters were widest in SBA media (1,6 mm) and in HBA media (0,8 mm). The characteristics of colony in 24 and 48 hours observation were not significantly different in all media. Conclusion HBA is an acceptable alternative media for culturing Streptococcus pneumoniae in poorly resourced laboratories. Keywords: Streptococcus pneumoniae, SBA, HBA PermaLink | Plain Format | Corresponding Author (Anita Oktari)

4	ABS-179	Biology
GROWTH ANALYSIS OF MICROSPORUM CANIS USING HUSK RICE (ORYZA SATIVA L. CV. CIHERANG) AS A REPLACEMENT FOR MEDIA SABOUROUD DEXTROSE AGAR (SDA) Haidir Syafrullah (1), Anita Oktari (2), Yati Supriatin (2), Muhamad Husni (2) (1) Medical School of Dharma Husada Bandung Indonesia (2) Department of Medical Analyst, School of Analyst Bakti Asih, Bandung Indonesia Abstract Rice husk is hard layer covering kariopsis which consists of two parts called the lemma and palea are interconnected in the milling process. Chaff is categorized biomass that can be used for a variety of needs such as raw materials and animal feed. Rice husk containing a source of carbohydrates and proteins is one of the factors that support the growth of mold. Sabouroud dextrose Agar (SDA) is the standard medium used for the growth of fungi. Experimental research has been carried out by inoculating Microsporum canis in alternative media flour rice husks. This study aimed to compare the growth of Microsporum canis in alternative media and media control. It has been done with varying concentrations penenelitian tempum rice husk 4.5%. 5%. and 5.5% Observations made by 14 days, incubated at room temperature with macroscopic examination each day by measuring the colony diameter using calipers (with units cm). Microscopic examination covers the length of the long hyphae makrokonidia and Microsporum canis using a microscope equipped microokuler (with units of m). By doing staining using Lactophenol solution Cotton Blue (LPCB). Observations processed statistically using one-way ANOVA. From the results of the statistical test was concluded that rice husk powder concentration of 5.5% is the optimum concentration for the growth of Microsporum canis and can be used as a replacement media Sabouroud Dextrose Agar (SDA). Keywords: Microsporum canis, alternative media, SDA PermaLink | Plain Format | Corresponding Author (Anita Oktari)

5	ABS-194	Biology
INCREASING ANTHRAQUINONE COMPOUNDS ON LEAF CALLUS Morinda citrifolia (L.) USING CHITOSAN FROM SHELL OF SHRIMPS (Panaeus monodon) Widi Purwianingsih (a*), Adi Rahmat & Rossy Y Hidayat Departemen Pendidikan Biologi FPMIPA Universitas Pendidikan Indonesia. Jl, Dr. Setia Budhi no.229 Bandung Indonesia. *widipurwianingsih[at]upi.edu Abstract Elicitation is one method to increase the content of secondary metabolite compounds in plant tissues. Anthraquinone is one of the secondary metabolite compounds that have potential of being a medicinal agent and produced by plants. The purpose of this research is to increase anthraquinone content in Morinda citrifolia (L.) leaf callus using elicitation method with chitosan from shrimp shell extract (Penaeus monodon) as elicitors. M.citrifolia (L.) callus was obtained from young leaf explants grown on Murashige and Skoog (MS) medium with 2.4-D addition of 1.75 mg / L and Kinetin 1.5 mg / L aseptically. The second subculture of callus is elicited by elicitor of chitosan each of 0.5 ml. The concentration of elicitor used was 0, 0.5,1,1.5,2 and 2.5 mg / ml. Harvesting of elicitation result done on day 0, 2, 4 and day 8 after elicitation. The results showed that callus containing anthraquinone could be induced in MS medium with addition of 2,4-D 1.75 and Kinetin 1.5 mg / L. and addition of chitosan at some concentrations may increase anthraquinone. The highest anthraquinone content was produced at concentration 2 mg / ml and 4 days harvest time Keywords: Morinda citrifolia (L.), Elicitation, Elicitor, Chitosan, Anthraquinone PermaLink | Plain Format | Corresponding Author (Widi Purwaningsih)

6	ABS-202	Biology
Study of vegetable waste product as alternative artificial feed to life cycle of Hermetia illuncens Yayan Sanjaya, Suhara, Rita Sintawati and Mimin Nurjhani Universitas Pendidikan Indonesia Abstract Reseach about artificial food and life cycle of Hermetia illucens was conducted. Alternative of animal organic feed is definitely needed cause its safe, cheap and healty. An descriptive reseach was conducted by investigate H. Illucens fecundity and life cycyle with alternative feed contain of bran, leftover vegetables and milk. The result showed that female fecundity resulting 750 eggs per day in average. Length of life cycle is 29 until 41 days. range of first larva is 1 until 2 days with 0,1until 0,3 cm in length. range the second larvae is 4 until 6 days with 0,4 until 0,6 mm in length. the third instar is around 7 until 10 days with 0,65 until 1,0 cm in length. fourth Instar is around 11until 13 day with 1,1 until 2,4 cm in length. BSF pre pupae 2,1 until 2,5 cm in length with a range 15 until 23 days. BSF pupae 2,3 until 2,4 cm in length with a range 24 until 31 days. The adult is 29 until 41 days with 2,2 until 2,4 cm in length. This show organic material can be turn into BSf biomass. Keywords: Hermetia illucens, fecundity, life cycle, artificial feed PermaLink | Plain Format | Corresponding Author (Yayan Sanjaya)

7	ABS-220	Biology
Cytotoxicity effects of Leaf Extracts of Ciplukan (Physalis angulata; Solanaceae) on human blood and ovary cancer cell lines Topik Hidayat, Didik Priyandoko, Fajar Sukma Perdana, Anggi Meinawan Insan Universitas Pendidikan Indonesia Abstract Physalis angulata has identified by molecular phylogenetic analysis and the results showed genetically close related with an anticancer plant, Withania somnifera. The aims of this preliminary study was to evaluate cytotoxicity effects of leaf extract of P. angulata (LEP) either in powder or pasta form on cells viability, cells proliferation, and inhibition on human ovary cancer cell lines (SKOV3) and human blood cancer cell lines (HL60). Cytotoxicity effects was analysed by MTS Cell Proliferation Assay Kit. Doxorubicin was used as positive control in this experiment. Surprisingly, two treatments (powder and pasta) have different toxic effects. We found that IC50 and viability (LC50) for SKOV3 cell lines for powder was between 93 ug/ml and 187 ug/ml, and 187 ug/ml and 375 ug/ml for pasta. IC50 for HL60 cell lines was 23 ug/ml for powder and around 18 ug/ml for pasta. Cell viability (LC50) of HL60 treated with LEP showed that 23 ug/ml for powder or 46 ug/ml for pasta. These results suggested that LEP have antiproliferative and inhibition activities both on SKOV3 or HL60. Keywords: cancer cell line, Ciplukan, in vitro, Solanaceae PermaLink | Plain Format | Corresponding Author (Topik Hidayat)

8	ABS-221	Biology
BLOOD SUGAR CONDITIONS IN HYPERGLYCEMIA MICE AFTER GIVEN THE BISCUIT FROM KEPOK BANANA SKIN Hernawati, Trisnawati Ajeng Kartini, Didik Priyandoko Department of Biology Education, Universitas Pendidikan Indonesia Bandung, Indonesia 40154 Abstract Hyperglycemia is a condition in which the blood glucose level exceeds normal limits. Hyperglycemia is a clinical manifestation of diabetes disorder, a metabolic disorder characterized by resistance to insulin action, inadequate insulin secretion or both. Research has been conducted to determine blood sugar level of male mice after given the biscuit from Kepok banana skin (Musa paradisiaca L.). The experiment was conducted experimentally with completely randomized design (RAL) design with five treatments i.e. positive and negative control, Banana skin flour on levels 25%, 50%, and 75%. Each treatment was repeated as many as six tests. Prior to treatment the mice were induced with alloxan to create hyperglycemia conditions. The biscuit from banana skins were given for 14 days. The results of research that has been done shows that the administration of biscuit from Kepok banana skin could be reduce blood sugar levels of mice who have hyperglycemia. In addition, after given the biscuit from Kepok banana skin has no effect on body weight of hyperglycemia mice. The biscuit from Kepok banana skin on levels 75% can be consumed for people with hyperglycemia to lower blood sugar. Keywords: Blood sugar, Kepok banana skin, flour, hyperglycemia, mice PermaLink | Plain Format | Corresponding Author (Hernawati hernawati)

9	ABS-230	Biology
Indigenous Knowledge in Utilizing Natural Resources as Sustainable Development in Kebon Gedang District of Ciwaringin Cirebon Dian Permana Putri Universitas Swadaya Gunung Jati Abstract This study aims to find out the indigenous knowledge of the Ciwaringin society in managing natural resources for sustainable development as a indigenous identity. The research method used in this study is a qualitative approach. Data was obtained through field observations and direct interviews with the indigeneous society and five craftsmen. The results showed that the majority of the Kebon Gedang Ciwaringin society was batik artisans which only painted batik produced in this region. In colouring process of batik, dyeing and painting techniques are used. Natural dyes are obtained from the bark, rind and leaves that are easy to find such as Mahogany, Tegeran, Mango and Indigo. Indigenous knowledge is only popular among craftsmen who originally obtained from the ancestors. This colouring technique is reused by 85% of craftsmen and become a uniqueness of Ciwaringin batik. Although the production process of a sheet of batik cloth is longer than the use of synthetic dyes, it increases the selling price, beside that this method is believed to reduce the risk of environmental pollution. This step supports the creation of sustainable development. It is hoped that the values of indigenous knowledge can be used as references and enrich scientific knowledge and become constructive thinking contributions to formulate policies of environmental management and conservation. Keywords: indigenous knowledge,natural dyes batik, Ciwaringin, sustainable development PermaLink | Plain Format | Corresponding Author (Dian Permana Putri)

10	ABS-256	Biology Education
Student conception about the Quality of Packaged Water H. S. Nuryani (a*) [N. Y. Rustaman (a) & D Rochintaniawati (b)] a. The School of Postgraduate Studies of Universitas Pendidikan Indonesia Jalan Dr. Setiabudhi No. 299 Bandung 40154 Jawa Barat, Indonesia phone/ Fax. +6222-2001937 http://sps.upi.edu b. Department of Science Education Faculty of Mathematics and Science Education Universitas Pendidikan Indonesia Jl. Dr. Setiabudi 229 Bandung 40154 Jawa Barat, Indonesia phone. 022-2013163 / Fax. 022-2013651 Abstract Instead of pure state, water on earth contains variety of material, either dissolved or suspended, including microbes. Therefore before being consumed, water should be processed first to reduce levels of contaminants to a save level. Standard of water quality for household need (Permen No.492/MENKES/PER/IV/2010) is usually used as reference for drinking water requirement. This preliminary study was carried out to investigate students conception about packaged/ bottled water quality assurance produced by certain vocational schools as products of their students. Explanatory survey method was used in this study to detect their conception deeply. The respondense or participants of this study was ten graders from public vocational school in Subang and their teachers in Agribusiness course. Data were collected through observation, interview, and test for their preconception. Research results show that students knowledge about mineral water is not in line with their understanding about the water quality, and their teachers as well. Keywords: vocational school, microbiology, quality assurance, Escherichia coli, Pedagogical Content Knowledge (PCK) PermaLink | Plain Format | Corresponding Author (Hadistia Siti Nuryani)

11	ABS-5	Biology Education
Confirmatory factor analysis of critical thinking disposition test in university biology Jayanti Syahfitri1,2*, Harry Firman1, Sri Redjeki1, and Siti Sriyati1 UNIVERSITAS PENDIDIKAN INDONESIA Abstract The purpose of this study was to look at the construct validity of Critical Thinking Disposition (CTD) test in biology. Referring to Facione theory there are seven indicators of CTD: truth seeking, open mind, analicity, systematicity, self confidence, inquisitiveness, and maturity. The instrument used is 10 clumps of questions consisting of 7 questions that lead to the CTD. The test are presented in the form of cases relating to biological content and have been validated by experts. The study involved 526 respondents who are biology education students from two universities in Bengkulu with study level (year1, 2, 3 and 4). After confirmed with CFA the results show that CTD test has suitable construct validity. This result is supported by the value received from Convergent Validity which includes the factor loading value of more than 0.5, while the Composite Reliability (CR) and Average Variance Extract (AVE) values for seven indicators of CTD are truth seeking (CR=0.96, AVE=0.72), open mind (CR=0.94, AVE=0.61), analicity (CR=0.97, AVE=0.76), sistematicity (CR=0.96, AVE=0.69), self confidence (CR=0.98, AVE=0.83), inquisitiveness (CR=0.93, AVE=0.56) and maturity (CR=0.97, AVE=0.75). From the results can be concluded that the test has construct validity and high composite reliability to measure the CTD in biology. Keywords: CFA, construct validity, critical thinking disposition, biology PermaLink | Plain Format | Corresponding Author (JAYANTI SYAHFITRI)

12	ABS-60	Biology Education
Development of Digital Product Teaching Assessment Instruments For PreService Teacher Biology Sri Maryanti (a*), Dede Trie Kurniawan (b), adam malik (c) a) Pendidikan Biologi FTK UIN Sunan Gunung Djati Bandung Jl. A.H Nasution No.105 Kota Bandung *Sri.maryanti[at]uinsgd.ac.id b) FKIP Universitas Swadaya Gunung Jati Cirebon Jl.Perjuangan No.1 Kota Cirebon c) Pendidikan Fisika FTK UIN Sunan Gunung Djati Bandung Jl. A.H Nasution No.105 Kota Bandung Abstract This research is aimed to know the characteristic of biological digital resource assessment instrument to evaluate biological stop motion video biology, biology learning web with weebly.com domain, interactive multimedia biology in the form of crossword puzzle, wondershare quiz creator, infographic easl.ly, online questionnaire with google form and teaching media material with prezzi in knowing the quality and feasibility of digital learning materials made biology teacher candidates. The instrument of assessment of digital teaching materials to be developed is reviewed according to the evaluation of evaluation experts, media experts and linguists. This development research uses ADDIE model which consists of analysis, design, development, implementation and evaluation. The subject of the instrument assessor is the lecturer of the evaluation course of education and three expert lecturers consisting of one evaluation expert, one media expert, and one linguist. Review and input from this expert lecturer as instrument validation. The developed assessment instrument has characteristics capable of measuring the sound, music, narration, animation, writing, color and video presentation of the biological animation video; able to measure aspects of use, navigation system, graphic design, content, access ability, access speed and facet of functions on the web of learning chemistry; able to measure aspects of ease of navigation, content of cognition, presentation of assessment information, media integration, artistic and aesthetics as well as the overall function of interactive multimedia biology; able to measure the design aspects of the program database, user friendly rules, material conformity, program usage and program usage techniques. Until now there is no in-depth assessment and research on the assessment of digital resources for biology that is widely published both in Indonesia and internationally. Therefore, the researcher is interested to develop the right and valid instrument structure in assessing the feasibility and quality of digital teaching materials produced by prospective biology teacher students. These development outcomes are expected to be published in national and international journals and serve as a reference for other Departements Education to preparing biology teachers. Keywords: research development, assessment instruments, digital biology teaching materials PermaLink | Plain Format | Corresponding Author (sri maryanti)

13	ABS-74	Biology Education
Genetics in Socio Scientific Issues: Measuring rebuttal abilities in scientific argumentation. A Anisa(a), A Widodo(b), R Riandi(b), and M Muslim(c) a)Post Graduate of Universitas Pendidikan Indonesia. jalan Dr. Setiabudhi no.229 Bandung Indonesia b)Biology Education of Universitas Pendidikan Indonesia. Jalan Dr. Setiabudhi no.229 Bandung Indonesia c)Physic Education of Universitas Pendidikan Indonesia. Jalan Dr. Setiabudhi no.229 Bandung Indonesia Abstract Genetics is one of the most important concepts in understanding Biology. Even though it is now widely applied in various socio-scientific issues (SSI), oftentimes, it is also cause a debate in the society. Students argumentation in addressing SSI that arises can be used as an assessment to see how students think in supporting or denying their claims. The case study held on 21 high school students in one of West Java public school. The aims of the study are to see students rebuttal ability and the genetic concept they use to support their rebuttal. The results showed that among the 21 students, only 4 students were able to present rebuttal with data support in all SSI tested. As many as 55.56% of students have not been able to provide rebuttal. 15.87% of students were able to provide rebuttal without data, and 26.98% of students were able to provide rebuttal with supporting data. Supporting data provided is only 14% indicating a linkage with genetic material. It shows that it is important to do the learning that support the growth of rebuttal ability in the learning process so that students argumentation ability becomes better, especially in understanding of the genetic concepts. Keywords: genetics, socio scientific issues, argumentation, rebuttal PermaLink | Plain Format | Corresponding Author (ANISA ROHANDI)

14	ABS-80	Biology Education
The effect of project based information in problem based learning on students scientific literacy in learning human excretory system Siti Juleha (a*), Dr. Selly Feranie, M.Si. (b), Ikmanda Nugraha, S.Pd., M.Pd (a) (a) International Program on Science Education Program Study, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia, Indonesia. (b) Physics Department, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia *Corresponding authors email address: siti.juleha95[at]student.upi.edu Abstract The present study aims to investigate the effect of project based information in Problem Based Learning on students scientific literacy of grade 8 studying in one of private schools in Bandung in learning human excretory system. A sample of 39 students in two classes was selected purposively from the five classes available in the school. Experimental group comprising 19 students received the instruction by Problem Based Learning with the final assessment of project based information while the control group comprising 20 students received the human excretory instruction by using Problem Based Learning without project based information in the final assessment. The data was collected via the pre test and post test administration. The results were statistically analyzed using SPSS software by employing independent t test. Results indicated that after the one-month intervention period, students in experimental group have higher score in the scientific literacy test compared to the students in the control group even it was not significantly different. It is concluded that project based information in problem based learning is useful to conduct as the learning strategies in the classroom. Keywords: project based information, problem based learning, scientific literacy PermaLink | Plain Format | Corresponding Author (Siti Juleha)

15	ABS-81	Biology Education
The Development of Science Comic in Human Digestive System Topic for Junior High School Nelah Roswati (a*), Nuryani Y. Rustaman(b), and Ikmanda Nugraha (a) a) International Program on Science Education Department, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia Jalan Setiabudhi No. 229, Isola, Sukasari, Bandung 40154, West Java, Indonesia *nelahroswati96[at]gmail.com b) Biology Department, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia Abstract The engagement of science education has been covered by using media, the popular media that usually used is printed media. One of popular printed media that actually most accessible and may be used in science education classroom as instructional tool is comic book. However, it is sometimes difficult to find the ideal and the appropriate comic books that can be used as the instructional tool of science education, because most of them are inappropriate for learning science and did not fit with the culture of the readers. The purpose of this study is to develop and evaluate the science comic aiming to contribute science learning about human digestive system topic concepts. In this research, a science comic book were created and implemented to 92 students of year eight from three different junior high schools and three science teachers as a subject implementation development. Student responses through the questionnaire and students-teachers readability test sheets were evaluated through qualitative content analysis. According to the result, most of the students believed that science comic book help learning through simplifying science concept and understanding the topic easier. Science comic book also help them to learning science more enjoy because the presence of humor and uniqueness aspect of the science comic book. Keywords: Instructional Media, Science Comic Book, Human Digestive System Topic PermaLink | Plain Format | Corresponding Author (Nelah Roswati)

16	ABS-349	Biology Education
Reducing cognitive load to improve quantitative literacy through implementing a worksheet based on Vee Diagram E. Nuraeni, A. Rahmat, S. A. Soesilawaty, I Permana, and A.C. Utami Universitas Pendidikan Indonesia Abstract This study aims to explore students cognitive load to improve quantitative literacy through implementing a worksheet based on Vee Diagram. This study was involved 31 students in 7th grade taking by purposive sampling. Three instruments were applied to measured students cognitive load, task complexity worksheet was used to asses students processing information ability which represent Intrinsic Cognitive Load (ICL), subjective rating scale questionnaire was used to asses students mental effort which represent Extraneous Cognitive Load (ECL), and paper based test was applied to asses students quantitative literacy which represent Germane Cognitive Load (GCL). A experiment worksheet based on Vee Diagram framework implemented in 2 weeks. Based on result show that ICL belong to very good category, ECL belong to low category, and GCL belong to good category. Correlation and regression test was applied to describe the relationship among three components of cognitive load. The results of correlation test shows that ICL, ECL, and GCL have a significant correlation (&#961; < 0.05). The result of regression test show that low condition of ICL and ECL have a positive contribution to GCL. It show that students cognitive load during learning process is low. Keywords: Cognitive load, literacy quantitative, Vee Diagram PermaLink | Plain Format | Corresponding Author (Eni Nuraeni)

17	ABS-354	Biology Education
Environmental Literacy Mapping Based On Adiwiyata and Non Adiwiyata at Junior High School in Ponorogo Siti Nurwaqidah 1,a) , and Suciati 2, Murni Ramli 3,b) Sebelas Maret University 1Faculty of Teacher Training and Education, Sebelas Maret University (nuwaqida[at]gmail.com) 2Faculty of Teacher Training and Education, Sebelas Maret University(suciati.sudarisman[at]yahoo.com) 3Faculty of Teacher Training and Education, Sebelas Maret University (mramlim[at]fkip.uns.ac.id) Abstract This research is used to find out the effectiveness of adiwiyata program between national, provincial, district and non-adiwiyata schools on environmental literacy based on age and gender. The research was conducted at junior high school in march-october 2017. Type of research is ex post facto. Sampling technique used is Proportionate stratified random sampling technique. The assessment used the MSELS standard supported by observational data. The results show that the adiwiyata program in ponorogo as a whole is related to the increase of environmental literacy with the sig value. 0.000. The higher the adiwiyata level, the higher the literacy value of the environment. Ability of environmental literacy among junior high school students between the ages of 13, 14, and 15 years or more is not different, with sig value. 0.308. The literacy ability of junior high school students environment between male and female gender is different with the sig value. 0.004. For general environmental literacy assessment in ponorogo is still low that more than 51% of respondents did not reach the score at level 3 which is the standard level of environmental literacy. The conclusions of the study were the types of levels of adiwiyata and gender affecting environmental literacy. Keywords: Environmental Literacy, Environmental Education, Adiwiyata PermaLink | Plain Format | Corresponding Author (SITI NURWAQIDAH)

18	ABS-355	Biology Education
Utilization of the school environment as a learning resource to improve critical thinking skills and scientific attitudes D A M Muharam(1), A Munandar(2) and S Sriyati(2) (1)Biology Education Study Program, School of Postgraduate, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudi 229, Bandung, Indonesia (2)Department of Biology Education, Faculty of Mathematics and Science Education Universitas Pendidikan Indonesia, Jalan Dr. Setiabudi 229, Bandung, Indonesia Abstract This study aims to determine the effect of utilization of the school environment as a learning resource in improving critical thinking skill and scientific attitude. This study compare differences in the improvement of critical thinking skills and scientific attitudes between the agricultural and coastal environment. The research method used is quasi experiment method. This design used two experimental groups and two control groups. The experimental and control class I are the experimental and control classes located in the agricultural environment. Experimental and control class II are experiment and control class located in coastal environment. Data collection techniques in this study were conducted through tests. The conclusion of this research is the utilization of the school environment as a learning resource, both in the agricultural and coastal environments can improve critical thinking skill and scientific attitude. However, the improvement of critical thinking skill and scientific attitudes between the agricultural and coastal environment do not have a significant difference. Keywords: school environment, learning resource, critical thinking skill, scientific attitude PermaLink | Plain Format | Corresponding Author (Dede Abdul Mujib Muharam)

19	ABS-103	Biology Education
Problem-based learning skills and reflection skills of biology education students through problem-based online tutorial M Sekarwinahyu 1,2*, N Y Rustaman 1, A Widodo 1, Riandi 1 1 Program Studi Pendidikan IPA, Sekolah Pascasarjana, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229, Bandung 40154, Indonesia 2 Program Studi Pendidikan Biologi, Universitas Terbuka, Jl. Cabe Raya, Pondok Cabe, Pamulang, Tangerang Selatan 15418, Indonesia *tika[at]ecampus.ut.ac.id Abstract This Study aims to find out the problem-based learning skills and reflection skills of biology education students through the problem-based online tutorial model using Gibbs reflective Cycles on the topic of algae and moss. The study is part of development research that is program implementation phase that was conducted in the semester 2018.1, involving 24 biology education students participating in the Plant Development online tutorial. The study is conducted by giving discourse related to Algae and moss difference and ask students to do problem-based learning process (PBL) and reflect on what they have done in the PBL process. The instruments used were the rubric of PBL and reflection skills 5 scales (range 0-4). The results showed that the PBL skills score of biology education student is at 1.9 and the reflection skill score is at 2.65. From the 6 PBL components assessed, the ability to formulate the problem is the highest ability (score 2.9) and the ability to answer the problem formulation is the lowest ability (score 1.3). Meanwhile, from the 6 reflection components assessed, the ability to conclude is the highest ability (score 3.8) and the ability to give the action plan is the lowest ability (score 0.9). Keywords: Problem based learning, reflection, online tutorial, Gibbs Reflective Cycle PermaLink | Plain Format | Corresponding Author (Mestika Sekarwinahyu)

20	ABS-359	Biology Education
The Effect of Learning Biology-based Inquiry Learning to the Improvement of Students Critical Thinking in High School Yuhana, Maridi, Sri Dwiastuti SEBELAS MARET UNIVERSITY Abstract This research is to know the effect of learning biology-based inquiry learning to the improvement of students critical thinking. The research design is quantitatively descriptive by drawing conclusion through statistical analysis. It is conducted in State Senior High School 1 Sembalun on academic year 2017/2018 with the population of all students in X class of natural sciences and the taken sample is students in X class of Natural Sciences 1. Sample is determined by purposive sampling. Based on research result of quantitatively descriptive analysis, gotten a score of pretest average value is (46.14) and a score of critical thinking skills posttest is (79.31), while average value of gain is (0,42) with a middle category. The inferential analysis result shows tcount = (22.52), whereas ttable in the 5% significance level is (2.02). Therefore, tcount> from ttable of 5%, thus Ha accepted, it means there is the significant effect between students pretest and posttest use learning biology-based inquiry learning. The research can be concluded that learning biology-based inquiry learning influence improvement of students critical thinking skill in class X of natural science. Keywords: Inquiry Learning, Critical Thinking PermaLink | Plain Format | Corresponding Author (YUHANA YUHANA)

21	ABS-110	Biology Education
The profile of the students scientific creative disposition in high school biology learning A Sukarso1,2,a), A Widodo1, D Rochintaniawati1, and W Purwianingsih1 1 Program Studi Pendidikan Ilmu Pengetahuan Alam, Sekolah Pascasarjana, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229, Bandung 40154, Indonesia 2 Program Studi Pendidikan Biologi FKIP Universitas Mataram, Jl. Majapahit No.62, Mataram 83125, Indonesia Abstract The purpose of this research is to identify the students scientific creative disposition and the position of creative disposition toward their peers in biology learning. Research subjects were 148 high school students in West Nusa Tenggara and West Java Provinces. The data were collected using a closed questionnaire technique. The collected data were analyzed qualitatively to determine the categories of students scientific creative disposition and quantitatively using t-test to determine the effect of scientific creative disposition on the position of scientific creative disposition against their peers. The results showed that the characteristics of students scientific creative disposition were categorized as low, average, and high. The domain of collaboration of the most prominent of scientific creative disposition of the domain inquisitive, persistent, imaginative and disciplined.The position of students scientific creative disposition toward their colleagues, 29.2 per cent influenced by the scientific creative disposition. This research contributes to develop students scientific creativity in high school biology learning. Keywords: Creative disposition, position of students creative disposition, and Biology science creativity PermaLink | Plain Format | Corresponding Author (Aa Sukarso)

22	ABS-136	Biology Education
Improving Scientific Argumentation : Opportunities and Barriers Analysis in Inquiry based Reading Riezky Maya Probosari*, Fatma Widyastuti, Sajidan, Suranto, Baskoro Adi Prayitno Science Education Doctoral Program Universitas Sebelas Maret Jl. Ir. Sutami No. 36A Surakarta Jawa Tengah-Indonesia *riezkymaya[at]fkip.uns.ac.id Abstract Reading is an inseparable part of science inquiry as a strategy for concept understanding and building knowledge through literature. Related to scientific argumentation, reading provides a very strong basis in terms of accuracy and validity of data, evidence and theory underlying a scientific statement. In many cases, lecturers face several challenges to effectively integrate argumentation into their classroom instruction. The purpose of this case study was to examine opportunities and barriers of the development of 2nd year college students scientific argumentation. The practice of argumentation as part of scientific communication is conducted over a semester on continuous topic using inquiry based reading instructions. This classroom based action research was held in one of the biology education study program in Central Java, Indonesia. Data sources included class discussion concentrated on individual presentation that occurred in nine weeks periods, students portfolio, questionnaire, and lecturer field notes. The opportunities and barriers of inquiry based reading was analysed. The results showed that there are many students who lack understanding of scientific reading strategies and how to use the information obtained to build strong arguments. Scientific reading culture is still very low, though through habituation, this can be resolved. Teaching strategies for doing inquiry based reading in practice are discussed, and the implications of these findings are highlighted in relation to develop sharper insight of scientific argumentation in science classroom. Keywords: scientific argumentation, inquiry-based reading, biology, teacher candidates PermaLink | Plain Format | Corresponding Author (Riezky Maya Probosari)

23	ABS-171	Biology Education
Application of Active-Cooperative Learning Model LSA Type on Improved Senior High School Students Learning Outcomes in the Human Immune System Sub-concept Reni Ernawati (a*), Uus Toharudin (b), Yusuf Ibrahim (b) a) Master of Biology Education Study Program, School of Postgraduate Studies, Universitas Pendidikan Indonesia *reniernawati17[at]student.upi.edu b) Department of Biology Education, Faculty of Teacher Training and Education, Universitas Pasundan Abstract This study aims to know the student learning outcomes by applying active-cooperative learning model Listen-Say-Arrange (LSA) type in the human immune system sub-concept. The method used is Quasi-Experimental, using Static Group Pre-test Post-test Design for the research design. Research participant consists of two classes of grade 11 science students in Bandung, which is grouped into control class and experimental class. Instrument used in this research is a multiple-choice test given through pre-test and post-test. Data analysis show that in control class which uses the conventional learning model (lecture method), the average value for pre-test is 52.62 and for post-test is 68.91. While in experimental class which uses active-cooperative learning LSA type, the average value for pre-test is 56.58 and for post-test is 77.58. T-test value for pre-test and post-test in control class is tcount(4.85) > t0.01(66)(2.66) and in experimental class is tcount(8.54) > t0.01(72)(2.65), both show significant differences. From the calculation of N-Gain obtained result in medium category, N-Gain in the control class is 0.34 and for experimental class is 0.48. Based on the results, can be concluded that the application of active-cooperative learning model LSA type in the human immune system sub-concept can improve students learning outcomes. Keywords: LSA; Learning outcomes; Human immune system PermaLink | Plain Format | Corresponding Author (Reni Ernawati)

24	ABS-224	Biology Education
Developing POE Based Learning to conceptual change of student in human respiratory concept K Kusnadi, Z. Lazuardi, and W. Surakusumah Departemen Pendidikan Biologi, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229, Bandung 40154, Indonesia Abstract The study to determine effect of Predict Observe Explain (POE) based learning on the conceptual change of high school students on the human respiratory system has been done. This study is a weak experimental of one group pretest and posttest design. The data were obtained by a pencil and paper test in the form of reasonable multiple choice questions. The data were analysed by mean of difference test between pretest and posttest scores. Based on study finding shows that a significant change in the percentage of scientific conceptions of students between pretest and posttest. The result shows that there is an increase conceptual change of student with average of N gain is 0.52 (moderate category). In addition, the results also shows that a pattern of conceptual change that positive change is 44.3%, positive survival is 20.5%, negative change is 11% and negative survival is 24.2% respectively. Based on result shows that POE-based learning model can be developed in senior high school to support students in overcoming conceptual change of scientific concept. Keywords: POE based learning, conceptual change, human respiratory system. PermaLink | Plain Format | Corresponding Author (K Kusnadi)

25	ABS-225	Biology Education
Developing POE-Based Learning to conceptual change of student in human respiratory concept K Kusnadi, Z. Lazuardi, and W. Surakusumah Departemen Pendidikan Biologi, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229, Bandung 40154, Indonesia Abstract The study to determine effect of Predict-Observe-Explain (POE)-based learning on the conceptual change of high school students on the human respiratory system has been done. This study is a weak experimental of one group pretest and posttest design. The data were obtained by a pencil and paper test in the form of reasonable-multiple choice questions. The data were analysed by mean of difference test between pretest and posttest scores. Based on study findings shows that a significant change in the percentage of scientific conceptions of students between pretest and posttest. The result shows that there is an increase conceptual change of student with average of N-gain is 0.52 (moderate category). In addition, the results also shows that a pattern of conceptual change that positive change is 44.3%, positive survival is 20.5%, negative change is 11% and negative survival is 24.2% respectively. Based on result shows that POE-based learning model can be developed in senior high school to support students in overcoming conceptual change of scientific concept. Keywords: POE based learning, conceptual change, human respiratory system PermaLink | Plain Format | Corresponding Author (K Kusnadi)

26	ABS-242	Biology Education
DEVELOPMENT OF THREE-TIER DIAGNOSTIC TEST INSTRUMENTS FOR DETECTING STUDENT CONCEPTION A.H. Anwar(a*), N.Y. Rustaman(b), and W. Purwianingsih(b) a) Program Studi Pendidikan Biologi, Sekolah Pascasarjana, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229, Bandung 40154, Indonesia * anggahidayatulanwar[at]gmail.com b) Departemen Pendidikan Biologi, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229, Bandung 40154, Indonesia Abstract A research and developmental study about developing three tier test instrument was carried out to detect students conception on cell concept. Students pre-conception about cell concept, cell reproduction and gametogenesis are often not in line with scientific concepts. This preliminary study was conducted to analyze the profile of students pre-conception about cell reproduction in senior high schools. Modified Borg and Galls R&D research design used in this research. Instrument development resulted in three tier diagnostic test in the form of 30 multiple choice test items with its reason and certainty index. To validate the instrument, it was administered towards a number of senior high school students (XI and XII graders) in Bandung. Then the response of the students were quantitatively analyzed. Research findings show that the instrument as product is effective to determine students conception, including their misconception. The percentage of misconception found in cell concepts mostly in gametogenesis (17.35%), compared to those who comprehend (27.83%), and not comprehend (54.82%). The results of this preliminary study will then be used for further study in preparing STEM based learning program which will facilitate 12th grade students on conceptual change in cell reproduction, as well as to prevent its misconception. Keywords: Three-tier diagnostic test, Student Conception, Cell PermaLink | Plain Format | Corresponding Author (Angga Hidayatul Anwar)

27	ABS-259	Chemistry
Effect of Temperature on Alpha-Cellulose from Rendement of Tobacco Stem Extraction Result Sri Seno Handayani, Amrullah, Husnul Fatimah and Rona Seftiani Mataram University Abstract One of Indonesias natural resources that have not been widely used is natural fiber or cellulose from tobacco. Previous study revealed tobacco stem contains significant level of cellulose, around 50%. Cellulose extraction process produces different rendement level, depending on the type of raw material and treatment used. This also means purity level of cellulose produced to be different. This paper presents the effect of temperature variations on tobacco stem rendement level and alfa-cellulose content. Extraction of tobacco stem was conducted using base method. The results showed that the highest cellulose yield was 76.25% with alfa-cellulose levels of 82% at temperature of 150^oC Keywords: cellulose, tobacco stem, extraction, temperature PermaLink | Plain Format | Corresponding Author (Sri Seno Handayani)

28	ABS-330	Chemistry
Adsorption of Iodine on Physical Activated and Chemical Modified Carbon Nanoporous as Controlled Drug Release Agent Candidate Maria Christina Prihatiningsih (a*), Fikri Ahmad Furqan (b), Imam Prasetyo (c), Teguh Ariyanto (c*) a) Nuclear Chemical Engineering of Polytechnic Institute of Nuclear Technology, National Nuclear Agency, Yogyakarta, Indonesia b) Chemical Engineering Derpartement of Gadjah Mada University, Yogyakarta, Indonesia c) The Center for Nuclear Fuel Technology, National Nuclear Agency, Serpong Tangerang, Indonesia *The Corresponding Authors Abstract Abstract. Adsorption of iodine have been performed on the physical activated and chemical modified carbon nanoporous as drug release agent candidate. Research has been conducted to find out the properties of iodine adsorption on activated carbon nanoporous material as preliminary test for iodine-131 adsorption. The purpose of this study was to determined the adsorption isotherm model, thermodynamic parameters, kinetic model and the value of activation energy. Nanoporous carbon material physically activated with steam and chemically modified with H2SO4. The concentration of iodine in filtrate after adsorption was analyzed by UV-Vis spectrophotometer. The experimental results have shown that the adsorption of iodine on physical activated and chemical modified carbon nanoporous were following the Langmuir adsorption isotherm modelwith the biggest Qmax value on physical activation was 111.11 mg/g and on chemical modification was 200 mg/g. Evaluation of thermodynamic parameters showed that iodine adsorption on physical activated and chemical modified carbon were exothermic with physical adsorption, occured spontaneously and less randomly. In addition, iodine adsorption on physical activated and chemical modified carbon nanoporous materials followed the pseudo second order kinetics model. The activation energy of iodine adsorption on physical activated carbon nanoporous was 18.02 kJ/mole and chemical modified activated carbon nanoporous was 20.54 kJ/mole. Physical activation and chemical modified carbon nanoporous had potential as drug release agents for iodine. Keywords: adsorption, nanoporous carbon, iodine PermaLink | Plain Format | Corresponding Author (Maria Christina Prihatiningsih)

29	ABS-384	Chemistry
Characterization and physicochemical properties of chlorophyll extract from Spirulina sp. Heli Siti Halimatul Munawaroh*1, M. Fathur R.1, Gun Gun Gumilar 1, Siti Aisyah 1, Ahmad Mudzakir 1, Galuh Yuliani 1, Asri Peni Wulandari2 1Program Studi Kimia, FPMIPA Universitas Pendidikan Indonesia Jl. Dr. Setiabudhi No. 229, Bandung 40154, Jawa Barat-Indonesia 2Departemen Biologi, Universitas Padjadjaran Jl. Raya Bandung-Sumedang, Hegarmanah, Jatinangor, Sumedang 45363, Jawa Barat-Indonesia Abstract Spirulina sp. is a group of microalgae which has a number of pigments with diverse bioactivities. Chlorophyll is one of the dominant pigments in Spirulina sp. that play an important role in capturing light in photosynthesis, which helps them to get energy from light. This study aims at investigating the characteristic of chlorophyll extract from Spirulina sp. and its physicochemical properties to light (white, yellow, and UV-C) and metal ions (Sn2+, K+, and Fe3+). The green spots with RF 0.45 and 0.37 values were observed in the TLC experiment, indicating the chlorophyll a and b, respectively. The presence of chlorophyll was also confirmed with UV-Vis and FTIR which detected the peak absorbance and typical absorption cluster for chlorophyll. The results show the chlorophyll was less resistance to all kinds of light (white, yellow, and UV-C). Metal ions of Sn2+, K+, and Fe3+ exhibited no obvious effect on the chlorophyll stability in the dark condition while in the light conditions the same metal ions influence obviously Keywords: Spirulina sp., pigment, Chlorophyll, phsicochemical properties, photosatbility, metal ion stability PermaLink | Plain Format | Corresponding Author (heli siti halimatul munawaroh)

30	ABS-393	Chemistry
Effect of UV C irradiation on secondary metabolites profile and antioxidant activity of germinated brown rice Siti Aisyah*, Annisa Nurjanah, Heli Siti Halimatul Munawaroh, Zackiyah Department of Chemistry Education, Universitas Pendidikan Indonesia, Bandung, Indonesia Abstract This study aims to investigate the effect of UV C irradiation on the secondary metabolites profile and the antioxidant activity of brown rice during two days germination. The change on the metabolite profile was identified using UPLC-ESI-QTOF, while antioxidant activity was performed using DPPH assay. The UPLC-ESI-QTOF chromatogram showed that three new peaks were found only in the germinated brown rice (GBR) that was irradiated by UV C. Based on the mass spectroscopy data, one of those three compounds was tentatively identified as glycosylated flavonoid, while the rest remained undetermined. The antioxidant activity of brown rice increased after two days germination, whilst there were no differences between antioxidant activities of germinated brown rice with UV-C irradiation and that of without UV-C irradiation. Keywords: UV C radiation, metabolites, brown rice PermaLink | Plain Format | Corresponding Author (Siti Aisyah)

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