Characterization and physicochemical properties of chlorophyll extract from Spirulina sp.
Heli Siti Halimatul Munawaroh*1, M. Fathur R.1, Gun Gun Gumilar 1, Siti Aisyah 1, Ahmad Mudzakir 1, Galuh Yuliani 1, Asri Peni Wulandari2

1Program Studi Kimia, FPMIPA Universitas Pendidikan Indonesia
Jl. Dr. Setiabudhi No. 229, Bandung 40154, Jawa Barat-Indonesia
2Departemen Biologi, Universitas Padjadjaran
Jl. Raya Bandung-Sumedang, Hegarmanah, Jatinangor, Sumedang 45363, Jawa Barat-Indonesia

Abstract

Spirulina sp. is a group of microalgae which has a number of pigments with diverse bioactivities. Chlorophyll is one of the dominant pigments in Spirulina sp. that play an important role in capturing light in photosynthesis, which helps them to get energy from light. This study aims at investigating the characteristic of chlorophyll extract from Spirulina sp. and its physicochemical properties to light (white, yellow, and UV-C) and metal ions (Sn2+, K+, and Fe3+). The green spots with RF 0.45 and 0.37 values were observed in the TLC experiment, indicating the chlorophyll a and b, respectively. The presence of chlorophyll was also confirmed with UV-Vis and FTIR which detected the peak absorbance and typical absorption cluster for chlorophyll. The results show the chlorophyll was less resistance to all kinds of light (white, yellow, and UV-C). Metal ions of Sn2+, K+, and Fe3+ exhibited no obvious effect on the chlorophyll stability in the dark condition while in the light conditions the same metal ions influence obviously

Keywords: Spirulina sp., pigment, Chlorophyll, phsicochemical properties, photosatbility, metal ion stability

Topic: Chemistry