THE OPTIMIZATION OF HUMAN BLOOD AGAR (HBA) FOR STREPTOCOCCUS PNEUMONIAE GROWTH Anita Oktari, Noviana Vanawati, Ulva Indah Kurniawati
Department of Medical Analyst, School of Analyst Bakti Asih, Bandung Indonesia
Abstract
Sheep Blood Agar (SBA) is a standard media for microbiology examination. The difficulty to get sheep blood in tropical and developing countries causes an obstacle in culturing Streptococcus pneumoniae. In other side, the usage of Human Blood Agar (HBA) were not recommended for culturing Streptococcus pneumoniae. The purpose of this experimental is to get the alternative media from HBA to improve the growth of Streptococcus pneumoniae. Methods experimental study with True experimental post test only design. Colony of Streptococcus pneumoniae that had been checked purity were planted on HBA media and SBA media. Observation was done in 24 hours and 48 hours, comprising of colony diameters, hemodygest diameters, and the characteristics colony. Results after 24 hours, the mean of Streptococcus pneumoniae colony diameters on SBA media were not significantly different (0,8 mm) with HBA media (0,6 mm). On the 48 hours observation, the mean of colony diameters on HBA media (1,5 mm) were narrower than those on SBA media (1,8 mm). After 24 hours, the mean of Streptococcus pneumoniae hemolysis diameters, on SBA media (1,2 mm) were significantly different with HBA media (0,6 mm). After the 48 hours observation, hemolysis diameters were widest in SBA media (1,6 mm) and in HBA media (0,8 mm). The characteristics of colony in 24 and 48 hours observation were not significantly different in all media. Conclusion HBA is an acceptable alternative media for culturing Streptococcus pneumoniae in poorly resourced laboratories.
