GROWTH ANALYSIS OF MICROSPORUM CANIS USING HUSK RICE (ORYZA SATIVA L. CV. CIHERANG) AS A REPLACEMENT FOR MEDIA SABOUROUD DEXTROSE AGAR (SDA)
Haidir Syafrullah (1), Anita Oktari (2), Yati Supriatin (2), Muhamad Husni (2)

(1) Medical School of Dharma Husada Bandung Indonesia
(2) Department of Medical Analyst, School of Analyst Bakti Asih, Bandung Indonesia

Abstract

Rice husk is hard layer covering kariopsis which consists of two parts called the lemma and palea are interconnected in the milling process. Chaff is categorized biomass that can be used for a variety of needs such as raw materials and animal feed. Rice husk containing a source of carbohydrates and proteins is one of the factors that support the growth of mold. Sabouroud dextrose Agar (SDA) is the standard medium used for the growth of fungi. Experimental research has been carried out by inoculating Microsporum canis in alternative media flour rice husks. This study aimed to compare the growth of Microsporum canis in alternative media and media control. It has been done with varying concentrations penenelitian tempum rice husk 4.5%. 5%. and 5.5% Observations made by 14 days, incubated at room temperature with macroscopic examination each day by measuring the colony diameter using calipers (with units cm). Microscopic examination covers the length of the long hyphae makrokonidia and Microsporum canis using a microscope equipped microokuler (with units of m). By doing staining using Lactophenol solution Cotton Blue (LPCB). Observations processed statistically using one-way ANOVA. From the results of the statistical test was concluded that rice husk powder concentration of 5.5% is the optimum concentration for the growth of Microsporum canis and can be used as a replacement media Sabouroud Dextrose Agar (SDA).

Keywords: Microsporum canis, alternative media, SDA

Topic: Biology