Isolation and Identification of Potential Probiotics Bacteria from Intestinal of Anguilla bicolor Peristiwati*, Yayan Sanjaya **and Canthika Trinaya
Department of Biology, Universitas Pendidikan Indonesia, Bandung, Indonesia
Abstract
Sidat is high selling price fish in international market. This is can causes sidat fish to be categorized as a source earners for the country. Probiotics are live bacteria promoted healthy in fish. Probiotics can also produce bacteriocins is usefull against several strains of pathogenic bacteria. This research aimed to isolated of probiotic bacteria in Anguila bicolor intestine and determine the characteristics of potential probiotics bacteria. The steps was taking sample of small intestine by dissect the stomach of Anguilla bicolor and taking out the digesta of small intestine and then dilution techniques were performed with pour plate and streak plate methods, Gram staining, Biochemical Tests and making Bacteria Growth Curve. To get the ability as probiotic bacteria by using a few test such as the ability in low pH and bile salt. The test of antagonistic ability to pathogen bacteria was used Staphylococcus aureus and Pseudomonas aeruginosa. The result shows that three isolates of Gram positive and have a rod shape. The three isolates characterized by biochemical tests belong to the genus Lactobacillus. They were able to grow and survive on a medium which has a pH between 3-6.5 and a medium of 0.05% and 0,1% synthetic bile salt. From the result of the antagonistic ability test show that all isolates have the ability to produce antimicrobial that are bacteriosida against bacterial growth of Staphylococcus aureus and Pseudomonas aeruginosa
